Methods and Materials
Before any experiments could be performed, SK-N-SH cells were cultured. Once at 100 percent confluency, they were ready to begin treatment. 0.2mL Retinoic acid was added to two dishes. 0.2 mL of control was added to the two other dishes. After 11 days, some cells were stained with methylene blue, and others with immunofluorescent to observe morphological changes. Treated cells were also tested for conductivity.
|